Escherichia coli TS

Ancestors

Derived strains

Genotype with respect to parental

△trpR, △tnaAB

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA added | △trpR, △tnaAB

Bars (|) indicate differences between strains.

Plasmids

pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA (P_tac-aroG^S211F -P_tac-trpE^{Q71K/S94N/C465Y} -trpABCD-serA, lacI)

Modifications

Gene	Organism	Modification
trpR	Escherichia coli	Silencing
tnaA	Escherichia coli	Silencing
tnaB	Escherichia coli	Silencing
trpE	Escherichia coli	Overexpression
trpD	Escherichia coli	Overexpression
trpC	Escherichia coli	Overexpression
trpB	Escherichia coli	Overexpression
trpA	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Scale	Ref.
L-tryptophan	Titer	5.99 g/L ^*	33.7 OD₆₀₀^*	Glucose	Flask	[ 258 ]
L-tryptophan	Titer	5.43 g/L ^*	20.54 OD₆₀₀^*	Glucose	Flask	[ 258 ]
L-tryptophan	Substrate yield	0.1 g/g of sustrate ^*	20.54 OD₆₀₀^*	Glucose	Flask	[ 258 ]

* Inferred from plots using RetroPlot.

References

Hou, Minglei; Gao, Shengqi; Wu, Jing; Chen, Sheng & Zhang, Kang. Metabolic engineering of Escherichia coli to enhance L-tryptophan biosynthesis. Systems Microbiology and Biomanufacturing. 2025.