Escherichia coli GPT101

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli GPT100

Escherichia coli GPT101

Derived strains

Genotype with respect to parental

ΔtrpL ΔpTrp::5CPtacs

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpR::FRT, ΔtnaA::FRT, ΔptsG::FRT | ΔtrpL ΔpTrp::5CPtacs

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
trpL	Escherichia coli	Silencing
trpA	Escherichia coli	Overexpression
trpB	Escherichia coli	Overexpression
trpC	Escherichia coli	Overexpression
trpD	Escherichia coli	Overexpression
trpE	Escherichia coli	Overexpression

References

Pengfei Gu, Fan Yang, Junhua Kang, Qian Wang & Qingsheng Qi (2012). One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in Escherichia coli. Microbial Cell Factories.

Pengfei Gu, Fan Yang, Fangfang Li, Quanfeng Liang & Qingsheng Qi (2013). Knocking out analysis of tryptophan permeases in Escherichia coli for improving L-tryptophan production. Applied Microbiology and Biotechnology.