Escherichia coli ZH-3

Ancestors

Derived strains

Genotype with respect to parental

P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | △trpR, △tnaAB | P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A | P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A | P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
aroG	Escherichia coli	Feedback insensitive
trpE	Escherichia coli	Feedback insensitive
trpD	Escherichia coli	Overexpression
trpC	Escherichia coli	Overexpression
trpB	Escherichia coli	Overexpression
trpA	Escherichia coli	Overexpression
serA	Escherichia coli	Feedback insensitive

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan	Substrate yield	0.18 g/g of sustrate		Glucose	35 h	Fed-Batch	[258]
L-tryptophan	Titer	43.0 g/L		Glucose	35 h	Fed-Batch	[258]
L-tryptophan	Volumetric productivity	1.23 g/L/h		Glucose		Fed-Batch	[258]
L-tryptophan	Titer	5.0 g/L ^*	19.36 OD₆₀₀^*	Glucose		Flask	[258]
L-tryptophan	Substrate yield	0.092 g/g of sustrate ^*	19.36 OD₆₀₀^*	Glucose		Flask	[258]
L-tryptophan	Titer	5.3 g/L ^*	20.73 OD₆₀₀^*	Glucose		Flask	[258]
L-tryptophan	Titer	5.1 g/L ^*	20.49 OD₆₀₀^*	Glucose		Flask	[258]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Hou, Minglei; Gao, Shengqi; Wu, Jing; Chen, Sheng & Zhang, Kang. Metabolic engineering of Escherichia coli to enhance L-tryptophan biosynthesis. Systems Microbiology and Biomanufacturing. 2025.