Escherichia coli HL-2

Ancestors

Derived strains

Genotype with respect to parental

△poxB

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | △trpR, △tnaAB | P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A | P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A | P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A | △poxB

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
poxB	Escherichia coli	Silencing

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan		Titer	5.08 g/L ^*	19.79 OD₆₀₀^*	Glucose		Flask	[ 258 ]

* Inferred from plots using RetroPlot.

References

Hou, Minglei; Gao, Shengqi; Wu, Jing; Chen, Sheng & Zhang, Kang. Metabolic engineering of Escherichia coli to enhance L-tryptophan biosynthesis. Systems Microbiology and Biomanufacturing. 2025.