Escherichia coli HL-3

Ancestors

Genotype with respect to parental

△tnaB:: P_J23109-prs^L135I

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | △trpR, △tnaAB | P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A | P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A | P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A | △poxB | △tnaB:: P_J23109-prs^L135I

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
prsA	Escherichia coli	Overexpression

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan		Titer	5.39 g/L ^*	20.39 OD₆₀₀^*	Glucose		Flask	[ 258 ]

* Inferred from plots using RetroPlot.

References

Hou, Minglei; Gao, Shengqi; Wu, Jing; Chen, Sheng & Zhang, Kang. Metabolic engineering of Escherichia coli to enhance L-tryptophan biosynthesis. Systems Microbiology and Biomanufacturing. 2025.