Escherichia coli HK overexpressed
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pJBEI-6409 added | Plasmid EcoCTs-CPMS5-glk addedBars (|) indicate differences between strains.
Plasmids
-
EcoCTs-CPMS5-glk (ColE1 ori, glk)
pJBEI-6409 (PUV5-AtoB-saHMGS-saHMGR-T1ter-Ptrc-MK-PMK-PMD-idi-TB0015-Ptrc-trGPPS-tLS-p15Aori-camR-lacI)
Production
| Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|---|
| (4S)-limonene |
|
Biomass-specific yield | 1291.7 µM/gDCW * | Glucose | 6 h * | Flask | [ 260 ] | |
| (4S)-limonene |
|
Biomass-specific yield | 1812.5 µM/gDCW * | Glucose | 7 h * | Flask | [ 260 ] |
* Inferred from plots using RetroPlot.