Escherichia coli ispALaFS-SN
Ancestors
Genotype with respect to wild type
F−, endA1, hsdR17 (r K −, m K + ), supE44, thi-l, λ−, recA1, gyrA96, ΔlacU169 (Φ80lacZ ΔM15) | Plasmid pTispALaFS added | Plasmid pSSN12Didi addedBars (|) indicate differences between strains.
Plasmids
-
pSSN12Didi (mvaK1Sp, mvaK2Sp, mvaDSp, idiEc)
pTispALaFS (Ptrc-RBS-ispA-linker (GGGGS)-aFS)
Production
Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
---|---|---|---|---|---|---|---|---|
(E,E)-α-farnesene |
|
Titer | 86.8 mg/L | Glycerol mevalonate | 48 h | Flask | [ 261 ] | |
(2E,6E)-farnesol |
|
Titer | 0.0 mg/L | Glycerol mevalonate | 48 h | Flask | [ 261 ] | |
(E,E)-α-farnesene |
|
Titer | 153.6 mg/L | Glycerol mevalonate | Flask | [ 261 ] | ||
(E,E)-α-farnesene |
|
Titer | 9.8 mg/L | Glycerol | Flask | [ 261 ] |
* Inferred from plots using RetroPlot.