Escherichia coli VHY16

Ancestors

Derived strains

Escherichia coli VHY17

Genotype with respect to parental

ΔldhA, ΔpflB, ΔadhE

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI, ycdN::P_trc-alsS_Bs | ydeU::P_trc-alsS | yjiV::P_trc-ilvED | ycgH::P_trc-ilvC | yjiT::P_trc-brnFE | yeeP::P_trc-spoT^{[R290E, K292D]} | ΔldhA, ΔpflB, ΔadhE

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
ldhA	Escherichia coli	Silencing
pflB	Escherichia coli	Silencing
adhE	Escherichia coli	Silencing

References

Yanan Hao, Qian Ma, Xiaoqian Liu, Xiaoguang Fan, Jiaxuan Men, Heyun Wu, Shuai Jiang, Daoguang Tian, Bo Xiong & Xixian Xie (2020). High-yield production of L-valine in engineered Escherichia coli by a novel two-stage fermentation. Metabolic Engineering.