Escherichia coli LEU12
Ancestors
Genotype with respect to parental
ΔlivJ
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | ycjV::Ptrc-leuAfbrEc | yciQ::Ptrc-leuAfbrEc | yghE::Ptrc-leuAfbrEc | yjgX::Ptrc-leuBCD | yjiT::Ptrc-ilvIHfbr | ylbE::Ptrc-ilvIHfbr | yjiV::Ptrc-ilvEDC | PleuA::Ptrc | ΔlivK | ΔlivJBars (|) indicate differences between strains.
Modifications
Gene | Organism | Modification |
---|---|---|
Silencing
|
Production
Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
---|---|---|---|---|---|---|---|
L-leucine | Titer | 8.45 g/L | 35.6 OD600* | Flask | [265] | ||
L-leucine | Substrate yield | 0.07 g/g of sustrate * | 35.6 OD600* | Flask | [265] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.