Escherichia coli LEU25

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli W3110 ΔlacI

Escherichia coli LEU03

Escherichia coli LEU04

Escherichia coli LEU05

Escherichia coli LEU06

Escherichia coli LEU07

Escherichia coli LEU08

Escherichia coli LEU09

Escherichia coli LEU10

Escherichia coli LEU11

Escherichia coli LEU14

Escherichia coli LEU16

Escherichia coli LEU17

Escherichia coli LEU20

Escherichia coli LEU21

Escherichia coli LEU22

Escherichia coli LEU23

Escherichia coli LEU24

Escherichia coli LEU25

Derived strains

Genotype with respect to parental

P_sucAB::P_esaS

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | ycjV::P_trc-leuA^fbr_Ec | yciQ::P_trc-leuA^fbr_Ec | yghE::P_trc-leuA^fbr_Ec | yjgX::P_trc-leuBCD | yjiT::P_trc-ilvIH^fbr | ylbE::P_trc-ilvIH^fbr | yjiV::P_trc-ilvEDC | P_leuA::P_trc | ΔlivK | livJ::P_trc-azlC | brnQ::P_trc-brnFE | yjiP::pntAB | ilvE::P_trc-Bcldh | ΔpoxB | ΔpflB | ΔldhA | yeeP::P_apFAB104-esaR | P_sucAB::P_esaS

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
sucA	Escherichia coli	Attenuation
sucB	Escherichia coli	Attenuation

References

Hao, Yanan; Pan, Xuewei; Li, Guomin; You, Jiajia; Zhang, Hengwei; Yan, Sihan; Xu, Meijuan & Rao, Zhiming. Construction of a plasmid-free l-leucine overproducing Escherichia coli strain through reprogramming of the metabolic flux. Biotechnology for Biofuels and Bioproducts. 2023, 16(1).