Escherichia coli LEU27
Ancestors
Genotype with respect to parental
trpR::Pbs2-esaI
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | ycjV::Ptrc-leuAfbrEc | yciQ::Ptrc-leuAfbrEc | yghE::Ptrc-leuAfbrEc | yjgX::Ptrc-leuBCD | yjiT::Ptrc-ilvIHfbr | ylbE::Ptrc-ilvIHfbr | yjiV::Ptrc-ilvEDC | PleuA::Ptrc | ΔlivK | livJ::Ptrc-azlC | brnQ::Ptrc-brnFE | yjiP::pntAB | ilvE::Ptrc-Bcldh | ΔpoxB | ΔpflB | ΔldhA | yeeP::PapFAB104-esaR | PsucAB::PesaS | trpR::Pbs2-esaIBars (|) indicate differences between strains.
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Silencing
|
||
|
Overexpression
|
Production
| Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|---|
| L-leucine | Titer | 55.0 g/L | 60.73 OD600* | Glucose | 48 h * | Fed-Batch | [ 265 ] | |
| L-leucine | Substrate yield | 0.23 g/g of sustrate | 60.73 OD600* | Glucose | 48 h * | Fed-Batch | [ 265 ] | |
| L-leucine | Titer | 16.25 g/L | 27.75 OD600* | Glucose | Flask | [ 265 ] | ||
| L-leucine | Substrate yield | 0.2 g/g of sustrate | 27.75 OD600* | Glucose | Flask | [ 265 ] |
* Inferred from plots using RetroPlot.