Escherichia coli HGC1

Ancestors

All strains

Escherichia coli K12

Escherichia coli WSH-Z06

Escherichia coli HGA1

Escherichia coli HGB6

Escherichia coli HGC1

Derived strains

Genotype with respect to parental

dadX-cvrA::P_j231119-yddG, tyrP::P_j231119-tktA, trpE::P_j231119-ppsA

Genotype with respect to wild type

F⁺ (λ) | l-Tyriosine auxotrophic | Plasmid pAP11 added | ΔtyrR, pheA and trpE | Plasmid pAP31 added | Plasmid pAP11 lost | ΔaroP and tyrP | Plasmid pAP11 added | Plasmid pAP31 lost | dadX-cvrA::P_j231119-yddG, tyrP::P_j231119-tktA, trpE::P_j231119-ppsA

Bars (|) indicate differences between strains.

Plasmids

pAP11 (pAP with aroG^fbr, tyrA^fbr, tktA and ppsA, p15A ori with P_RP_L)

Modifications

Gene	Organism	Modification
dadX	Escherichia coli	Silencing
cvrA	Escherichia coli	Silencing
yddG	Escherichia coli	Overexpression
tyrP	Escherichia coli	Silencing
tktA	Escherichia coli	Overexpression
trpE	Escherichia coli	Silencing
ppsA	Escherichia coli	Overexpression

References

Ping, Jurong; Wang, Lian; Qin, Zhijie; Zhou, Zhemin & Zhou, Jingwen. Synergetic engineering of Escherichia coli for efficient production of l-tyrosine. Synthetic and Systems Biotechnology. 2023, 8(4), 724-731.