Escherichia coli HGC1
Ancestors
Derived strains
Genotype with respect to parental
dadX-cvrA::Pj231119-yddG, tyrP::Pj231119-tktA, trpE::Pj231119-ppsA
Genotype with respect to wild type
F+ (λ) | l-Tyriosine auxotrophic | ΔtyrR, pheA and trpE | Plasmid pAP11 added | ΔaroP and tyrP | Plasmid pAP11 lost | Plasmid pAP31 added | dadX-cvrA::Pj231119-yddG, tyrP::Pj231119-tktA, trpE::Pj231119-ppsA | Plasmid pAP31 lost | Plasmid pAP11 addedBars (|) indicate differences between strains.
Plasmids
-
pAP11 (pAP with aroGfbr, tyrAfbr, tktA and ppsA, p15A ori with PRPL)
Modifications
| Gene | Organism | Modification |
|---|---|---|
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Silencing
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Silencing
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Overexpression
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Silencing
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Overexpression
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Silencing
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Overexpression
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Production
| Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|---|
| L-tyrosine | Titer | 4.03 g/L * | 13.18 OD600* | Glucose | Flask | [ 268 ] | ||
| L-tyrosine | Titer | 4.12 g/L * | 14.0 OD600* | Glucose | Flask | [ 268 ] |
* Inferred from plots using RetroPlot.