Escherichia coli HGC4
Ancestors
Derived strains
Genotype with respect to parental
ykgH-betA::Pj231119-udhA, yeeJ-yeeL::Pj231119-pntAB
Genotype with respect to wild type
F+ (λ) | l-Tyriosine auxotrophic | Plasmid pAP11 added | ΔtyrR, pheA and trpE | Plasmid pAP31 added | Plasmid pAP11 lost | ΔaroP and tyrP | Plasmid pAP11 added | Plasmid pAP31 lost | dadX-cvrA::Pj231119-yddG, tyrP::Pj231119-tktA, trpE::Pj231119-ppsA | Plasmid pAP33 added | Plasmid pAP11 lost | ykgH-betA::Pj231119-udhA, yeeJ-yeeL::Pj231119-pntABBars (|) indicate differences between strains.
Plasmids
-
pAP33 (pAP with aroGfbr, tyrAfbr, fpkBa and pta, p15A ori with PRPL)
Modifications
| Gene | Organism | Modification |
|---|---|---|
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Silencing
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Silencing
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Overexpression
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Silencing
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Silencing
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Overexpression
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Overexpression
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Production
| Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|---|
| L-tyrosine | Titer | 6.17 g/L | Glucose | 48 h * | Flask | [ 268 ] | ||
| L-tyrosine | Titer | 50.2 g/L | Glucose | 69 h | Fed-Batch | [ 268 ] | ||
| acetate | Titer | 12.4 g/L | Glucose | 69 h | Fed-Batch | [ 268 ] |
* Inferred from plots using RetroPlot.