Escherichia coli HGD3
Ancestors
Derived strains
Genotype with respect to parental
ΔpoxB
Genotype with respect to wild type
F+ (λ) | l-Tyriosine auxotrophic | ΔtyrR, pheA and trpE | Plasmid pAP11 added | ΔaroP and tyrP | Plasmid pAP11 lost | Plasmid pAP31 added | dadX-cvrA::Pj231119-yddG, tyrP::Pj231119-tktA, trpE::Pj231119-ppsA | Plasmid pAP31 lost | Plasmid pAP11 added | Plasmid pAP11 lost | Plasmid pAP33 added | ykgH-betA::Pj231119-udhA, yeeJ-yeeL::Pj231119-pntAB | ΔpoxBBars (|) indicate differences between strains.
Plasmids
-
pAP33 (pAP with aroGfbr, tyrAfbr, fpkBa and pta, p15A ori with PRPL)
Strain derived from a randomly mutated strain.
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Silencing
|
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-tyrosine | Titer | 6.5 g/L * | 13.73 OD600* | Flask | [268] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.