Escherichia coli HGD (M9)

Ancestors

Genotype with respect to wild type
F+ (λ) | l-Tyriosine auxotrophic | Plasmid pAP11 added | ΔtyrR, pheA and trpE | Plasmid pAP31 added | Plasmid pAP11 lost | ΔaroP and tyrP | Plasmid pAP11 added | Plasmid pAP31 lost | dadX-cvrA::Pj231119-yddG, tyrP::Pj231119-tktA, trpE::Pj231119-ppsA | Plasmid pAP33 added | Plasmid pAP11 lost | ykgH-betA::Pj231119-udhA, yeeJ-yeeL::Pj231119-pntAB | ΔpoxB

Bars (|) indicate differences between strains.


Plasmids

  • pAP33 (pAP with aroGfbr, tyrAfbr, fpkBa and pta, p15A ori with PRPL)


Strain generated through random mutagenesis


Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
L-tyrosine Titer 7.11 g/L Glucose 48 h Flask [ 268 ]
L-tyrosine Titer 80.5 g/L Glucose 72 h Fed-Batch [ 268 ]
L-tyrosine Substrate yield 0.278 g/g of sustrate Glucose 72 h Fed-Batch [ 268 ]
L-tyrosine Titer 92.8 g/L Glucose 62 h Fed-Batch [ 268 ]
L-tyrosine Substrate yield 0.266 g/g of sustrate Glucose 62 h Fed-Batch [ 268 ]
acetate Titer 9.0 g/L Glucose 62 h Fed-Batch [ 268 ]

* Inferred from plots using RetroPlot.


References