Escherichia coli HGD

Synonyms

Escherichia coli M9

Ancestors

Genotype with respect to wild type
F+ (λ) | l-Tyriosine auxotrophic | ΔtyrR, pheA and trpE | Plasmid pAP11 added | ΔaroP and tyrP | Plasmid pAP11 lost | Plasmid pAP31 added | dadX-cvrA::Pj231119-yddG, tyrP::Pj231119-tktA, trpE::Pj231119-ppsA | Plasmid pAP31 lost | Plasmid pAP11 added | Plasmid pAP11 lost | Plasmid pAP33 added | ykgH-betA::Pj231119-udhA, yeeJ-yeeL::Pj231119-pntAB | ΔpoxB

Bars (|) indicate differences between strains.


Plasmids

  • pAP33 (pAP with aroGfbr, tyrAfbr, fpkBa and pta, p15A ori with PRPL)


Strain generated through random mutagenesis.


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-tyrosine Titer 7.11 g/L 48 h Flask [268]
L-tyrosine Titer 80.5 g/L 72 h Fed-Batch [268]
L-tyrosine Substrate yield 0.278 g/g of sustrate 72 h Fed-Batch [268]
L-tyrosine Titer 92.8 g/L 62 h Fed-Batch [268]
L-tyrosine Substrate yield 0.266 g/g of sustrate 62 h Fed-Batch [268]
acetate Titer 9.0 g/L 62 h Fed-Batch [268]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References