Escherichia coli 5HV9
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid pCDF-Trc-yajO-hok/sok added | Plasmid pSTV-Plac-T7rbs-davBA-gabT-yqhD-gabP-lysP-hok/sok addedBars (|) indicate differences between strains.
Plasmids
-
pSTV-Plac-T7rbs-davBA-gabT-yqhD-gabP-lysP-hok/sok (davBA-gabT-yqhD-gabP-lysP-hok/sok)
pCDF-Trc-yajO-hok/sok (Ptrc-yajO-hok/sok)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| 5-hydroxypentanoate | Titer | 5.59 g/L | 120 h | Flask | [269] | ||
| 5-hydroxypentanoate | Substrate yield | 0.96 mol/mol of substrate | 120 h | Flask | [269] | ||
| 5-hydroxypentanoate | Titer | 35.6 g/L | Batch | [269] | |||
| 5-hydroxypentanoate | Substrate yield | 0.92 mol/mol of substrate | Batch | [269] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.