Escherichia coli HM5 (pBRmetL–pNrhtA)
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | ΔlysA | ΔmetA | ΔthrBC | ΔtdcC | Plasmid pBRmetL–pNrhtA addedBars (|) indicate differences between strains.
Plasmids
-
pBRmetL–pNrhtA (metL, PN25-rhtA)
Production
Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
---|---|---|---|---|---|---|---|---|
L-homoserine | Titer | 39.54 g/L | 14.55 g/L | Glucose | 44 h | Fed-Batch | [ 272 ] | |
L-homoserine | Titer | 0.29 g/g of sustrate | 14.55 g/L | Glucose | 44 h | Flask | [ 272 ] | |
L-homoserine | Titer | 0.9 g/L/h | 14.55 g/L | Glucose | 44 h | Fed-Batch | [ 272 ] | |
acetate | Titer | 5.2 g/L | 14.55 g/L | Glucose | 44 h | Fed-Batch | [ 272 ] | |
acetate | Substrate yield | 0.038 g/L | 14.55 g/L | Glucose | 44 h | Fed-Batch | [ 272 ] |
* Inferred from plots using RetroPlot.