Escherichia coli BFPA2

Ancestors

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | ΔpheA ΔfeaB | Plasmid pETDuet-ARO10-yahK added | Plasmid pRSFDuet-aroA added

Bars (|) indicate differences between strains.

Plasmids

pRSFDuet-aroA (RSF1030 ori, lacI, P_T7lac-aroA)

pETDuet-ARO10-yahK (pBR322 origin, P_T7lac-RBS-ARO10, P_T7lac-RBS-yahK)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
4-tyrosol	Titer	2.16 mM		Glucose		Flask	[279]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Yang, Haiquan; Xue, Yuxiang; Yang, Cui; Shen, Wei; Fan, You & Chen, Xianzhong. Modular Engineering of Tyrosol Production in Escherichia coli. Journal of Agricultural and Food Chemistry. 2019, 67(14), 3900-3908.