Escherichia coli BFPE3

Ancestors

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | ΔpheA ΔfeaB | Plasmid pACYCDuet-aroA-tyrA* added | Plasmid pRSFDuet-ARO10-yahK added

Bars (|) indicate differences between strains.

Plasmids

pRSFDuet-ARO10-yahK (RSF1030 ori, lacI, P_T7lac-ARO10_Sc, P_T7lac-yahK_Ec)

pACYCDuet-aroA-tyrA* (p15A ori, lacI P_T7lac-aroA, P_T7lac-tyrA*)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
4-tyrosol	Titer	4.61 mM		Glucose		Flask	[279]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Yang, Haiquan; Xue, Yuxiang; Yang, Cui; Shen, Wei; Fan, You & Chen, Xianzhong. Modular Engineering of Tyrosol Production in Escherichia coli. Journal of Agricultural and Food Chemistry. 2019, 67(14), 3900-3908.