Escherichia coli BFPG3
Ancestors
Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | ΔpheA ΔfeaB | Plasmid pCDFDuet-aroE-aroD added | Plasmid pRSFDuet-ARO10-yahK added | Plasmid pETDuet-aroA-tyrA* addedBars (|) indicate differences between strains.
Plasmids
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pRSFDuet-ARO10-yahK (RSF1030 ori, lacI, PT7lac-ARO10Sc, PT7lac-yahKEc)
pETDuet-aroA-tyrA* (pBR322 origin, PT7lac-RBS-aroA, PT7lac-RBS-tyrA*)
pCDFDuet-aroE-aroD (CloDF13 ori, lacI, PT7lac-aroE, PT7lac-aroD)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| 4-tyrosol | Titer | 5.7 mM | Flask | [279] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.