Escherichia coli KW023

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid PH5a1 added | ΔtrpR ΔtrpL | ΔpykF | ΔptsH | M1-37::galP | M1-93::glk | Plasmid PH5a1 lost | Plasmid PH5a2 added

Bars (|) indicate differences between strains.


Plasmids

  • PH5a2 (aroGfbr trpEDCBA sRNA-pta)


Strain derived from a randomly mutated strain.


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
L-tryptophan Titer 39.7 g/L Fed-Batch [135]
L-tryptophan Volumetric productivity 1.6 g/L/h Fed-Batch [135]
L-tryptophan Substrate yield 0.167 g/g of sustrate 28 h Fed-Batch [135]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References