Escherichia coli BWZ3-Acs/Acc (pACYC-PhlD)

Ancestors

All strains

Escherichia coli B

Escherichia coli BL21

Escherichia coli BL21(DE3)

Escherichia coli BWZ1

Escherichia coli BWZ3

Escherichia coli BWZ3-Acs/Acc

Escherichia coli BWZ3-Acs/Acc (pACYC-PhlD)

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | ΔackA-pta | ΔadhE | Plasmid pACYC-FabF/Acs added | Plasmid pACYC-Acs added | Plasmid pACYC-FabF/Acs lost | Plasmid pACYC-Acs lost | Plasmid pACYC-PhlD added

Bars (|) indicate differences between strains.

Plasmids

pACYC-PhlD (phlD_Pf)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
phloroglucinol	Titer	1280.0 mg/L		Glucose		Flask	[198]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Ikuo Miyahisa, Masafumi Kaneko, Nobutaka Funa, Hisashi Kawasaki, Hiroyuki Kojima, Yasuo Ohnishi & Sueharu Horinouchi (2005). Efficient production of (2S)-flavanones by Escherichia coli containing an artificial biosynthetic gene cluster. Applied Microbiology and Biotechnology.