Escherichia coli WLGB-RPP/pTrcDx-idi-mdh, pLyc184
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | ΔgdhA | ΔgpmB | pTrc-ispA-dxs pTrc-idi | pTrc-pps | Plasmid pTrcDx-idi-mdh added | Plasmid pLyc184 addedBars (|) indicate differences between strains.
Plasmids
-
pLyc184 (crtEIB under gntT104 promoter)
pTrcDx-idi-mdh (Ptrc dxs idi mdh)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| all-trans-lycopene | Titer | 19.35 mg/L | 48 h | Flask | [155] | ||
| all-trans-lycopene | Biomass-specific yield | 10.63 mg/g DCW | 48 h | Flask | [155] | ||
| all-trans-lycopene | Biomass-specific productivity | 221.52 µg/g DCW/h | 48 h | Flask | [155] | ||
| all-trans-lycopene | Titer | 283.0 mg/L | 36 h | Fed-Batch | [155] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.