Escherichia coli LH06

Ancestors

Genotype with respect to wild type

recA1, thi, endA1, supE44, gyrA96, hsdR17 Δ(lac-proAB)/(lac-proAB)/F′[traD36,proAB+ , lacIq , lacZΔM15] | △tnaA::Km^R | △sdaA::Km^R | Plasmid pTrcACB added

Bars (|) indicate differences between strains.

Plasmids

pTrcACB (serA^fbr(H344A/N364A), serC, serB)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-cysteine	Titer	620.9 mg/L		Glucose	48 h	Flask	[209]
L-cysteine	Substrate yield	0.06 g/g of sustrate		Glucose	48 h	Flask	[209]
L-cysteine	Titer	5.1 g/L		Glucose	32 h	Fed-Batch	[209]
acetate	Titer	0.0 g/L ^*	0.96 g/L^*	Glucose	48 h ^*	Flask	[227]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Han Liu, Guochen Fang, Hui Wu, Zhimin Li & Qin Ye (2018). L‐Cysteine Production in Escherichia coli Based on Rational Metabolic Engineering and Modular Strategy. Biotechnology Journal.

Han Liu, Yehua Hou, Yu Wang & Zhimin Li (2019). Enhancement of Sulfur Conversion Rate in the Production of l‑Cysteine by Engineered Escherichia coli. Journal of Agricultural & Food Chemistry.