Escherichia coli Mgly13

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli MG1655(DE3)

Escherichia coli Mgly1

Escherichia coli Mgly13

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | fhuA2 | ΔldhA | Plasmid pJNU-3 added

Bars (|) indicate differences between strains.

Plasmids

pJNU-3 (P_trc-ycdW-aceA-aceK)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
glycolate	Substrate yield	0.385 g/g of sustrate		Glucose		Fed-Batch	[214]
glycolate	Titer	1.13 g/L		Glucose		Flask	[214]
glycolate	Substrate yield	0.385 g/g of sustrate		Glucose		Flask	[214]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Yu Deng, Ning Ma, Kangjia Zhu, Yin Mao, Xuetuan Wei & Yunying Zhao (2018). Balancing the carbon flux distributions between the TCA cycle and glyoxylate shunt to produce glycolate at high yield and titer in Escherichia coli. Metabolic Engineering.