Escherichia coli W3110 trpD9923/pJLBaroGfbr
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | trpD9923 | Plasmid pJLBaroGfbr addedBars (|) indicate differences between strains.
Plasmids
-
pJLBaroGfbr (lacIq, PlacUV5-aroGfbr)
Strain derived from a randomly mutated strain.
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| anthranilate | Titer | 0.44 g/L | 1.09 g/L | Flask | [151] | ||
| anthranilate | Titer | 6.72 g/L | 19.47 g/L | 38 h | Fed-Batch | [151] | |
| acetate | Titer | 3.18 g/L | 19.47 g/L | 38 h | Fed-Batch | [151] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.
References
- Víctor E Balderas-Hernández, Andrea Sabido-Ramos, Patricia Silva, Natividad Cabrera-Valladares, Georgina Hernández-Chávez, José L Báez-Viveros, Alfredo Martínez, Francisco Bolívar & Guillermo Gosset (2009). Metabolic engineering for improving anthranilate synthesis from glucose in Escherichia coli. Microbial Cell Factories.