Escherichia coli W3110 trpD9923PTS^-/pJLBaroG^fbrtktA

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli W3110 trpD9923

Escherichia coli W3110 trpD9923 PTS^-

Escherichia coli W3110 trpD9923PTS^-/pJLBaroG^fbrtktA

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | trpD9923 | ΔptsHI, crr::km^R | Plasmid pJLBaroG^fbrtktA added

Bars (|) indicate differences between strains.

Plasmids

pJLBaroG^fbrtktA (lacI^q, P_lacUV5-aroG^fbr, P_tktA-tktA)

Strain derived from a randomly mutated strain.

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
anthranilate	Titer	0.45 g/L	0.39 g/L	Glucose		Flask	[151]
anthranilate	Titer	7.16 g/L	11.12 g/L	Glucose	38 h	Fed-Batch	[151]
acetate	Titer	0.0 g/L	11.12 g/L	Glucose	38 h	Fed-Batch	[151]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Víctor E Balderas-Hernández, Andrea Sabido-Ramos, Patricia Silva, Natividad Cabrera-Valladares, Georgina Hernández-Chávez, José L Báez-Viveros, Alfredo Martínez, Francisco Bolívar & Guillermo Gosset (2009). Metabolic engineering for improving anthranilate synthesis from glucose in Escherichia coli. Microbial Cell Factories.