Escherichia coli MG1655 (pMIV-cysE5, pACYC-yhaM1)
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pMIV-cysE5 added | Plasmid pACYC-yhaM1 addedBars (|) indicate differences between strains.
Plasmids
-
pACYC-yhaM1 (yhaM)
pMIV-cysE5 (p15A ori, PompC-cysE5)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-cysteine | Titer | 14.9 mg/L | 22.0 OD600 | 24 h | Flask | [226] | |
| L-cysteine | Substrate yield | 0.037 % | 22.0 OD600 | 24 h | Flask | [226] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.