Escherichia coli LH12
Ancestors
Genotype with respect to wild type
recA1, thi, endA1, supE44, gyrA96, hsdR17 Δ(lac-proAB)/(lac-proAB)/F′[traD36,proAB+ , lacIq , lacZΔM15] | △tnaA::KmR | △sdaA::KmR | ΔyhaM::KanR | Ptrc1-serAfbrCB::KanR | PcysM::Ptrc1 | PnrdH::Ptrc1 | Plasmid pLH03 addedBars (|) indicate differences between strains.
Plasmids
-
pLH03 (PcysE-cysEfbr, PGAPDH-ydeD)
Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| L-cysteine | Titer | 1035.63 mg/L * | 1.5 g/L* | 48 h * | Flask | [227] | |
| acetate | Titer | 0.0 g/L * | 1.5 g/L* | 48 h * | Flask | [227] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.