Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH)

Ancestors

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | thrA^fbr | lysC^fbr | P_thr::P_tac | ΔlysA | ΔmetA | ilvA^C290T | Δtdh | iclR::Cm^R | P_ppc::P_trc | ΔCm^R | ilvA^{C1339T, G1341T, C1351G, T1352C} | P_ilvEDA::P_trc, P_ilvC::P_trc | P_lrp::P_trc | Plasmid pBRthrABCygaZH added | Plasmid pTacilvAIH added

Bars (|) indicate differences between strains.

Plasmids

pTacilvAIH (ilvAIH)

pBRthrABCygaZH (thrA^fbrBC ygaZH)

Production

Metabolites	Production type	Production	Carbon source	Time	Scale	Ref.
L-isoleucine	Titer	2.83 g/L	Glucose		Flask	[228]
L-isoleucine	Titer	8.72 g/L	Glucose		Fed-Batch	[228]
acetate	Titer	12.61 g/L	Glucose		Fed-Batch	[228]
L-isoleucine	Volumetric productivity	0.121 g/L/h	Glucose		Fed-Batch	[228]
L-isoleucine	Titer	9.46 g/L	Glucose	60 h	Fed-Batch	[228]
L-isoleucine	Volumetric productivity	0.158 g/L/h	Glucose	60 h	Fed-Batch	[228]
L-isoleucine	Substrate yield	0.14 g/g of sustrate	Glucose	60 h	Fed-Batch	[228]
acetate	Titer	11.34 g/L	Glucose	60 h	Fed-Batch	[228]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Jin Hwan Park, Jae Eun Oh, Kwang Ho Lee, Ji Young Kim & Sang Yup Lee (2012). Rational Design of Escherichia coli for l‑Isoleucine Production. ACS Synthetic Biology.