Titer in Fed-Batch for trans-4-hydroxy-L-proline

		Modifications
Strain	Maximum production	putA	putP	proP	aceA	proB	esaR	Datp4h	esaI	sucA	phyH
E. coli HYP0	8,800 mg/L
E. coli HYP3	15,400 mg/L
E. coli HYPA	15,700 mg/L
E. coli HYP1	27,300 mg/L
E. coli HYP2	43,200 mg/L
E. coli HYP2M	54,800 mg/L

Volumetric productivity in Fed-Batch for trans-4-hydroxy-L-proline

		Modifications
Strain	Maximum production	putA	putP	proP	aceA	proB	esaR	Datp4h	esaI	sucA	phyH
E. coli HYP3	60 mg/L/h
E. coli HYP0	210 mg/L/h
E. coli HYPA	300 mg/L/h
E. coli HYP1	650 mg/L/h
E. coli HYP2M	910 mg/L/h
E. coli HYP2	1,030 mg/L/h

Substrate yield in Fed-Batch for trans-4-hydroxy-L-proline

		Modifications
Strain	Maximum production	putA	putP	proP	aceA	proB	esaR	Datp4h	esaI	sucA	phyH
E. coli HYPA	0.05 g/g of sustrate
E. coli HYP0	0.05 g/g of sustrate
E. coli HYP1	0.12 g/g of sustrate
E. coli HYP2	0.18 g/g of sustrate
E. coli HYP2M	0.24 g/g of sustrate
E. coli HYP3	0.37 g/g of sustrate