Substrate yield in Fed-Batch for glycolate

		Modifications
Strain	Maximum production	ldhA	ghrA	aceA	aceK	icd	gltA	glcB	aceB	aldA
E. coli Mgly13	0.385 g/g of sustrate
E. coli Mgly134	0.504 g/g of sustrate
E. coli EYX-2	0.52 g/g of sustrate
E. coli Mgly434	0.765 g/g of sustrate

Substrate yield in Flask for glycolate

		Modifications
Strain	Maximum production	ldhA	ghrA	aceA	aceK	icd	gltA	glcB	aceB	aldA	glcD	glcE	glcF
E. coli BW25113-pGLY-1	0.0813 g/g of sustrate
E. coli EXY-1-pGLY-1	0.11 g/g of sustrate
E. coli Mgly12	0.189 g/g of sustrate
E. coli Mgly11	0.2 g/g of sustrate
E. coli Mgly12P	0.287 g/g of sustrate
E. coli Mgly13	0.385 g/g of sustrate
E. coli EYX-2	0.48 g/g of sustrate
E. coli Mgly134	0.534 g/g of sustrate
E. coli EYX-3	0.57 g/g of sustrate
E. coli Mgly534	0.679 g/g of sustrate
E. coli Mgly334	0.75 g/g of sustrate
E. coli Mgly434	0.79 g/g of sustrate

Substrate yield in Batch for glycolate

		Modifications
Strain	Maximum production	aceB	glcB	ghrA	aceA	aceK
E. coli EYX-2	0.48 g/g of sustrate

Titer in Flask for glycolate

		Modifications
Strain	Maximum production	ldhA	ghrA	aceA	aceK	icd	gltA	glcB	aceB	aldA	glcD	glcE	glcF
E. coli BW25113-pGLY-1	650 mg/L
E. coli EXY-1-pGLY-1	880 mg/L
E. coli Mgly12	950 mg/L
E. coli Mgly11	1,100 mg/L
E. coli Mgly13	1,130 mg/L
E. coli Mgly134	1,370 mg/L
E. coli Mgly534	1,800 mg/L
E. coli Mgly12P	1,960 mg/L
E. coli Mgly334	2,400 mg/L
E. coli EYX-1-glcb-1-pGLY-1	2,440 mg/L
E. coli EYX-2	2,660 mg/L
E. coli Mgly434	2,822 mg/L

Titer in Fed-Batch for glycolate

		Modifications
Strain	Maximum production	ldhA	glcB	aceB	aldA	ghrA	aceA	aceK	icd	gltA
E. coli EYX-2	56,440 mg/L
E. coli Mgly434	65,500 mg/L

Titer in Batch for glycolate

		Modifications
Strain	Maximum production	aceB	glcB	ghrA	aceA	aceK
E. coli EYX-2	8,960 mg/L