Titer in Flask for 5-aminolevulinate

		Modifications
Strain	Maximum production	hemA	sucC	hemB	aceA	coaA	ppc	rhtA	eamA	grxA	katG	sodA	trxC	msrA	msrB	katE	sodB	sodC
E. coli ALA1	1,225 mg/L
E. coli ALA2	1,358 mg/L
E. coli ALA4	1,358 mg/L
E. coli ALA5	1,383 mg/L
E. coli ALA6	1,400 mg/L
E. coli ALA3	1,408 mg/L
E. coli ALA7	1,508 mg/L
E. coli ALA8	1,830 mg/L
E. coli ALA9	2,440 mg/L
E. coli ALA21	3,143 mg/L
E. coli ALA17	3,905 mg/L
E. coli ALA10	4,050 mg/L
E. coli ALA11	4,910 mg/L
E. coli ALA12	5,390 mg/L
E. coli ALA18	6,190 mg/L
E. coli ALA25	6,834 mg/L
E. coli ALA16	6,857 mg/L
E. coli ALA15	7,048 mg/L
E. coli ALA19	7,720 mg/L
E. coli ALA20	7,830 mg/L
E. coli ALA22	7,890 mg/L
E. coli ALA13	8,008 mg/L
E. coli ALA23	8,235 mg/L
E. coli ALA24	8,539 mg/L
E. coli ALA14	9,790 mg/L

Titer in Fed-Batch for 5-aminolevulinate

		Modifications
Strain	Maximum production	coaA	sucC	hemB	aceA	hemA	ppc	eamA	grxA
E. coli ALA14	4,500 mg/L
E. coli ALA26	30,700 mg/L

Substrate yield in Flask for 5-aminolevulinate

		Modifications
Strain	Maximum production	hemA	sucC	hemB	aceA	coaA	ppc	rhtA	eamA	grxA	trxC	msrA	msrB	katE	katG	sodA	sodB	sodC
E. coli ALA1	0.14921 g/g of sustrate
E. coli ALA2	0.16086 g/g of sustrate
E. coli ALA5	0.16086 g/g of sustrate
E. coli ALA4	0.16232 g/g of sustrate
E. coli ALA6	0.16595 g/g of sustrate
E. coli ALA3	0.17105 g/g of sustrate
E. coli ALA7	0.18779 g/g of sustrate
E. coli ALA21	0.20162 g/g of sustrate
E. coli ALA8	0.2271 g/g of sustrate
E. coli ALA17	0.23219 g/g of sustrate
E. coli ALA9	0.26494 g/g of sustrate
E. coli ALA10	0.26494 g/g of sustrate
E. coli ALA12	0.31226 g/g of sustrate
E. coli ALA11	0.31298 g/g of sustrate
E. coli ALA16	0.31735 g/g of sustrate
E. coli ALA15	0.3239 g/g of sustrate
E. coli ALA13	0.3603 g/g of sustrate
E. coli ALA22	0.37631 g/g of sustrate
E. coli ALA19	0.38941 g/g of sustrate
E. coli ALA14	0.40688 g/g of sustrate
E. coli ALA20	0.4178 g/g of sustrate
E. coli ALA18	0.4571 g/g of sustrate

Substrate yield in Fed-Batch for 5-aminolevulinate

		Modifications
Strain	Maximum production	coaA	sucC	hemB	aceA	hemA	ppc	eamA	grxA
E. coli ALA26	0.38723 g/g of sustrate

Volumetric productivity in Fed-Batch for 5-aminolevulinate

		Modifications
Strain	Maximum production	coaA	sucC	hemB	aceA	hemA	ppc	eamA	grxA
E. coli ALA26	1,020 mg/L/h