Silencing
Overexpression
Feedback insensitive
Attenuation
Feed-forward insensitive
Inhibition insensitive
Unstated
Combined modifications
Titer in Fed-Batch for catechol
Modifications | |||||||
---|---|---|---|---|---|---|---|
Strain | Maximum production | trpD | aroG | tktA | antA | antB | antC |
E. coli W3110 trpD9923/pJLBaroGfbrtktA/pTrc-ant3 | 4,470 mg/L |
Titer in Flask for catechol
Modifications | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Strain | Maximum production | trpD | antA | antB | antC | aroG | tktA | ptsH | ptsI | crr |
E. coli W3110 trpD9923/pTrc-ant3 | 150 mg/L | |||||||||
E. coli trpD9923 PTS-/pTrc-ant3 | 370 mg/L | |||||||||
E. coli W3110 trpD9923/pJLBaroGfbrtktA/pTrc-ant3 | 410 mg/L |
Substrate yield in Fed-Batch for catechol
Modifications | |||||||
---|---|---|---|---|---|---|---|
Strain | Maximum production | trpD | aroG | tktA | antA | antB | antC |
E. coli W3110 trpD9923/pJLBaroGfbrtktA/pTrc-ant3 | 0.16 g/g of sustrate |
Substrate yield in Flask for catechol
Modifications | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Strain | Maximum production | trpD | ptsH | ptsI | crr | antA | antB | antC | aroG | tktA |
E. coli W3110 trpD9923/pTrc-ant3 | 0.05 g/g of sustrate | |||||||||
E. coli trpD9923 PTS-/pTrc-ant3 | 0.09 g/g of sustrate | |||||||||
E. coli W3110 trpD9923/pJLBaroGfbrtktA/pTrc-ant3 | 0.1 g/g of sustrate |
Biomass-specific productivity in Flask for catechol
Modifications | ||||||||
---|---|---|---|---|---|---|---|---|
Strain | Maximum production | trpD | ptsH | ptsI | crr | antA | antB | antC |
E. coli trpD9923 PTS-/pTrc-ant3 | 0.03 mg/L/g DCW |
Specific production rate in Flask for catechol
Modifications | |||||||
---|---|---|---|---|---|---|---|
Strain | Maximum production | trpD | antA | antB | antC | aroG | tktA |
E. coli W3110 trpD9923/pTrc-ant3 | 50 mg/g DCW/h | ||||||
E. coli W3110 trpD9923/pJLBaroGfbrtktA/pTrc-ant3 | 70 mg/g DCW/h |
Specific production rate in Fed-Batch for catechol
Modifications | |||||||
---|---|---|---|---|---|---|---|
Strain | Maximum production | trpD | aroG | tktA | antA | antB | antC |
E. coli W3110 trpD9923/pJLBaroGfbrtktA/pTrc-ant3 | 20 mg/g DCW/h |