Titer in Batch for (S)-malate

		Modifications
Strain	Maximum production	pta	pckA	pck	ldhA	adhE
E. coli W3110/p10499A	0 mg/L
E. coli W3110/p104ColiPck	0 mg/L
E. coli W3110/p104ManPck	0 mg/L
E. coli WGS-3/p10499A	0 mg/L
E. coli WGS-3/p104ColiPck	0 mg/L
E. coli WGS-10/p10499A	0 mg/L
E. coli WGS-10/p104ColiPck	1,420 mg/L
E. coli WGS-10/p104ManPck	9,250 mg/L
E. coli WGS-3/p104ManPck	9,620 mg/L

Titer in Flask for (S)-malate

		Modifications
Strain	Maximum production	ldhA	poxB	pflB	pta	ackA	NADP-ME2	frdB	frdC	fumB	fumA	fumC	POS5	frdA	frdD	ptsG	ppc	pckA	mdh	pncB	pyc
E. coli BA040	1,730 mg/L
E. coli F0501	1,970 mg/L
E. coli BA050	3,220 mg/L
E. coli BA041	3,530 mg/L
E. coli F0511	3,620 mg/L
E. coli BA042	4,390 mg/L
E. coli BA052	4,390 mg/L
E. coli BA051	5,300 mg/L
E. coli F0711	5,630 mg/L
E. coli F0811	6,870 mg/L
E. coli F0911	7,780 mg/L
E. coli BA062	9,190 mg/L
E. coli BA061	9,390 mg/L
E. coli BA063	13,140 mg/L
E. coli F0931	21,650 mg/L

Titer in Fed-Batch for (S)-malate

		Modifications
Strain	Maximum production	pflB	ldhA	fumB	frdA	frdB	frdC	frdD	ptsG	ppc	pckA	mdh
E. coli BA063	28,500 mg/L

Substrate yield in Batch for (S)-malate

		Modifications
Strain	Maximum production	pta	pckA	pck	ldhA	adhE
E. coli W3110/p10499A	0 g/g of sustrate
E. coli W3110/p104ColiPck	0 g/g of sustrate
E. coli W3110/p104ManPck	0 g/g of sustrate
E. coli WGS-3/p10499A	0 g/g of sustrate
E. coli WGS-3/p104ColiPck	0 g/g of sustrate
E. coli WGS-10/p10499A	0 g/g of sustrate
E. coli WGS-10/p104ColiPck	0.08064 g/g of sustrate
E. coli WGS-10/p104ManPck	0.41054 g/g of sustrate
E. coli WGS-3/p104ManPck	0.44719 g/g of sustrate

Substrate yield in Flask for (S)-malate

		Modifications
Strain	Maximum production	ldhA	poxB	pflB	pta	ackA	frdB	frdC	fumB	fumA	fumC	NADP-ME2	POS5	frdA	frdD	ptsG	ppc	pckA	mdh	pncB	pyc
E. coli BA040	0.2 g/g of sustrate
E. coli BA042	0.3 g/g of sustrate
E. coli BA041	0.34 g/g of sustrate
E. coli BA050	0.34 g/g of sustrate
E. coli F0931	0.36 g/g of sustrate
E. coli BA052	0.44 g/g of sustrate
E. coli BA051	0.46 g/g of sustrate
E. coli BA062	0.61 g/g of sustrate
E. coli BA061	0.63 g/g of sustrate
E. coli BA063	0.73 g/g of sustrate

Substrate yield in Fed-Batch for (S)-malate

		Modifications
Strain	Maximum production	pflB	ldhA	fumB	frdA	frdB	frdC	frdD	ptsG	ppc	pckA	mdh
E. coli BA063	0.69 g/g of sustrate

Volumetric productivity in Batch for (S)-malate

		Modifications
Strain	Maximum production	pta	pckA	pck	ldhA	adhE
E. coli W3110/p10499A	0 mg/L/h
E. coli W3110/p104ColiPck	0 mg/L/h
E. coli W3110/p104ManPck	0 mg/L/h
E. coli WGS-3/p10499A	0 mg/L/h
E. coli WGS-3/p104ColiPck	0 mg/L/h
E. coli WGS-10/p10499A	0 mg/L/h
E. coli WGS-10/p104ColiPck	90 mg/L/h
E. coli WGS-3/p104ManPck	690 mg/L/h
E. coli WGS-10/p104ManPck	740 mg/L/h

Volumetric productivity in Flask for (S)-malate

		Modifications
Strain	Maximum production	ldhA	poxB	pflB	pta	ackA	frdB	frdC	fumB	fumA	fumC	NADP-ME2	POS5
E. coli F0911	110 mg/L/h
E. coli F0931	300 mg/L/h

Biomass-specific yield in Flask for (S)-malate

		Modifications
Strain	Maximum production	ldhA	poxB	pflB	pta	ackA	NADP-ME2
E. coli F0501	190 mg/g DCW
E. coli F0511	410 mg/g DCW