Titer in Fed-Batch for 3-methyl-2-oxobutanoate

		Modifications
Strain	Maximum production	ackA	pta	pflB	adhE	frdA	ldhA	ilvE	leuA	pntA	pntB	ilvC	alsS	ilvD	aceF
E. coli 050T4/pCTSDT	46,400 mg/L
E. coli 050TY/pCTSDTQ487S-RBS55	55,800 mg/L

Titer in Flask for 3-methyl-2-oxobutanoate

		Modifications
Strain	Maximum production	ackA	pta	pflB	adhE	frdA	ldhA	ilvC	alsS	ilvD	ilvE	ilvA	leuA	pntA	pntB	aceF
E. coli B0016-050/pCTSDT	330 mg/L
E. coli BL21(DE3)/pCTSDT	1,700 mg/L
E. coli 050T1/pCTSDT	7,360 mg/L
E. coli 050T2/pCTSDT	7,390 mg/L
E. coli 050T4-1/pCTSDT	7,560 mg/L
E. coli 050T4-2/pCTSDT	7,690 mg/L
E. coli 050T/pCTSDT	8,010 mg/L
E. coli 050T3/pCTSDT	8,440 mg/L
E. coli 050T3/pCTSDT+pACYC-pntAB	9,350 mg/L
E. coli 050T4/pCTSDTQ487S	14,880 mg/L
E. coli 050T4/pCTSDT	19,200 mg/L
E. coli 050T4/pCTSDTQ487S-RBS55	19,200 mg/L
E. coli 050TY/pCTSDTQ487S-RBS55	22,000 mg/L

Volumetric productivity in Fed-Batch for 3-methyl-2-oxobutanoate

		Modifications
Strain	Maximum production	ackA	pta	pflB	adhE	frdA	ldhA	ilvE	leuA	pntA	pntB	ilvC	alsS	ilvD	aceF
E. coli 050T4/pCTSDT	1,780 mg/L/h
E. coli 050TY/pCTSDTQ487S-RBS55	2,140 mg/L/h

Specific production rate in Fed-Batch for 3-methyl-2-oxobutanoate

		Modifications
Strain	Maximum production	ackA	pta	pflB	adhE	frdA	ldhA	ilvE	leuA	pntA	pntB	ilvC	alsS	ilvD	aceF
E. coli 050T4/pCTSDT	112 mg/g DCW/h
E. coli 050TY/pCTSDTQ487S-RBS55	162 mg/g DCW/h

Substrate yield in Fed-Batch for 3-methyl-2-oxobutanoate

		Modifications
Strain	Maximum production	ackA	pta	pflB	adhE	frdA	ldhA	ilvE	leuA	pntA	pntB	ilvC	alsS	ilvD	aceF
E. coli 050T4/pCTSDT	0.41147 g/g of sustrate
E. coli 050TY/pCTSDTQ487S-RBS55	0.54437 g/g of sustrate

Substrate yield in Flask for 3-methyl-2-oxobutanoate

		Modifications
Strain	Maximum production	ackA	pta	pflB	adhE	frdA	ldhA	ilvE	leuA	pntA	pntB	ilvC	alsS	ilvD	aceF
E. coli 050T4/pCTSDT	0.50923 g/g of sustrate
E. coli 050TY/pCTSDTQ487S-RBS55	0.6006 g/g of sustrate