Silencing
Overexpression
Feedback insensitive
Attenuation
Feed-forward insensitive
Inhibition insensitive
Unstated
Combined modifications

Titer in Flask for L-valine

Modifications
Strain Maximum production lacIEc alsSBs ilvEEc ilvDEc ilvCEc brnFCg brnECg yeePEc spoTEc pntAEc pntBEc ilvBCg ilvHCg ygaZEc ygaHEc gapCEc relAEc ilvIEc ilvHEc ilvBEc ilvNEc ilvGEc ilvMEc ilvAEc bcdBs
E. coli W3110 0 mg/L
E. coli VHY01 300 mg/L
E. coli VHY02 4,650 mg/L
E. coli V1 cat-PL-bcd 5.7 9,100 mg/L
E. coli V1 cat-PL-ilvE5.7 9,800 mg/L
E. coli VHY03 10,500 mg/L
E. coli VHY04 10,500 mg/L
E. coli VHY05 16,530 mg/L
E. coli VHY06 18,600 mg/L
E. coli VHY09 18,875 mg/L
E. coli VHY08 19,000 mg/L
E. coli VHY07 19,125 mg/L
E. coli VHY12 25,770 mg/L
E. coli VHY11 31,875 mg/L
E. coli VHY10 32,000 mg/L
E. coli VHY13 39,100 mg/L
E. coli VHY14 41,200 mg/L
E. coli VHY15 41,300 mg/L

Titer in Fed-Batch for L-valine

Modifications
Strain Maximum production lacIEc alsSBs ilvEEc ilvDEc ilvCEc brnFCg brnECg yeePEc spoTEc ldhAEc pflBEc adhEEc bcdBs
E. coli VHY18 86,000 mg/L

Substrate yield in Flask for L-valine

Modifications
Strain Maximum production lacIEc alsSBs ilvEEc ilvDEc ilvCEc brnFCg brnECg yeePEc spoTEc pntAEc pntBEc ygaZEc ygaHEc gapCEc relAEc
E. coli VHY02 0.043 g/g of sustrate
E. coli VHY03 0.1 g/g of sustrate
E. coli VHY04 0.1 g/g of sustrate
E. coli VHY05 0.146 g/g of sustrate
E. coli VHY06 0.17 g/g of sustrate
E. coli VHY12 0.19 g/g of sustrate
E. coli VHY09 0.191 g/g of sustrate
E. coli VHY08 0.194 g/g of sustrate
E. coli VHY07 0.195 g/g of sustrate
E. coli VHY10 0.3 g/g of sustrate
E. coli VHY11 0.3 g/g of sustrate
E. coli VHY13 0.32 g/g of sustrate
E. coli VHY14 0.33 g/g of sustrate
E. coli VHY15 0.33 g/g of sustrate

Substrate yield in Fed-Batch for L-valine

Modifications
Strain Maximum production lacIEc alsSBs ilvEEc ilvDEc ilvCEc brnFCg brnECg yeePEc spoTEc ldhAEc pflBEc adhEEc bcdBs
E. coli VHY18 0.41 g/g of sustrate

Volumetric productivity in Fed-Batch for L-valine

Modifications
Strain Maximum production lacIEc alsSBs ilvEEc ilvDEc ilvCEc brnFCg brnECg yeePEc spoTEc ldhAEc pflBEc adhEEc bcdBs
E. coli VHY18 2,330 mg/L/h