Silencing
Overexpression
Feedback insensitive
Attenuation
Feed-forward insensitive
Inhibition insensitive
Unstated
Combined modifications

Titer in Flask for L-tyrosine

Modifications
Strain Maximum production tyrREc aroGEc tyrAEc ppsAEc tktAEc aroLEc tyrCZm tyrPEc aroPEc aroHEc tnaAEc tnaBEc pheAEc pheLEc trpEEc ydiBEc aroDEc aroBEc tyrBEc aroCEc aroAEc aroKEc aroEEc dadXEc cvrAEc yddGEc ykgHEc betAEc sthAEc yeeJEc yeeLEc pntAEc pntBEc xfpkBa ptaEc poxBEc ldhAEc pflBEc adhEEc fruKEc ackAEc
E. coli K12 ΔtyrR 0 mg/L
E. coli BTY0.3 0 mg/L
E. coli BTY0.4 0 mg/L
E. coli K12 ΔtyrR (pCL1920::aroGWT tyrAWT) 6 mg/L
E. coli K12 ΔtyrR (pCL1920::aroGfbr tyrAWT) 71 mg/L
E. coli K12 ΔtyrR (pCL1920::aroGWT tyrAfbr) 86 mg/L
E. coli K12 ΔtyrR (pCL1920::aroGfbr tyrAfbr) 127 mg/L
E. coli DPD4193 180 mg/L
E. coli T1 346 mg/L
E. coli K12 ΔtyrR (pCL1920::PLtetO−1 aroGfbr tyrAfbr tktA) 380 mg/L
E. coli HGD1 430 mg/L
E. coli K12 ΔtyrR (pCL1920::PLtetO−1aroGfbr tyrAfbr ppsA) 466 mg/L
E. coli BTY0.5 488.4 mg/L
E. coli BTY1.5 552.6 mg/L
E. coli T2 621 mg/L
E. coli HGD2 740 mg/L
E. coli A (pS0, pY0) 746 mg/L
E. coli BTY0.10 760.6 mg/L
E. coli WSH-Z06 (pAP13) 800 mg/L
E. coli BTY1.2 806 mg/L
E. coli B (pS0, pY1) 897 mg/L
E. coli E (pS4, pY2) 908 mg/L
E. coli BTY0.11 923 mg/L
E. coli BTY1.10 942.6 mg/L
E. coli BTY1.1 1,083.6 mg/L
E. coli WSH-Z06 (pAP22) 1,100 mg/L
E. coli C (pS4, pY1) 1,150 mg/L
E. coli WSH-Z06 (pAP01) 1,200 mg/L
E. coli D (pS5, pY1) 1,219 mg/L
E. coli BTY1.11 1,235 mg/L
E. coli BTY1.12 1,239 mg/L
E. coli WSH-Z06 (pAP01) ΔpflB 1,290 mg/L
E. coli G (pS5, pY3) 1,310 mg/L
E. coli WSH-Z06 (pAP01) ΔtrpE 1,340 mg/L
E. coli WSH-Z06 (pAP21) 1,400 mg/L
E. coli WSH-Z06 (pAP01) ΔldhA 1,470 mg/L
E. coli WSH-Z06 (pAP01) ΔpheA 1,470 mg/L
E. coli WSH-Z06 (pAP12) 1,500 mg/L
E. coli WSH-Z06 (pAP01) ΔadhE 1,670 mg/L
E. coli HGC2 1,780 mg/L
E. coli WSH-Z06 (pAP11) 1,800 mg/L
E. coli WSH-Z06 (pAP01) ΔtyrR,pheA,trpE 1,800 mg/L
E. coli BTY0.12 1,808 mg/L
E. coli BTY1.13 1,896 mg/L
E. coli WSH-Z06 (pAP01) ΔtyrR,trpE 1,980 mg/L
E. coli BTY0.13 2,004 mg/L
E. coli WSH-Z06 (pAP01) ΔtyrR 2,130 mg/L
E. coli F (pS4, pY3) 2,169 mg/L
E. coli WSH-Z06 (pAP01) ΔtyrR,pheA,adhE 2,190 mg/L
E. coli WSH-Z06 (pAP01) ΔtyrR,ldhA 2,270 mg/L
E. coli BTY7.13 2,483 mg/L
E. coli BTY2.13 2,567 mg/L
E. coli WSH-Z06 (pAP01) ΔtyrR,pheA,pflB 2,790 mg/L
E. coli WSH-Z06 (pAP01) ΔtyrR,adhE 3,080 mg/L
E. coli WSH-Z06 (pAP01) ΔtyrR,pheA,ldhA 3,180 mg/L
E. coli WSH-Z06 (pAP01) ΔtyrR,pheA 3,200 mg/L
E. coli HGA5 3,200 mg/L
E. coli HGA3 3,490 mg/L
E. coli HGA6 3,610 mg/L
E. coli WSH-Z06 (pAP01) ΔtyrR,pflB 3,680 mg/L
E. coli HGA4 3,700 mg/L
E. coli HGA2 3,850 mg/L
E. coli HGC1 4,120 mg/L
E. coli HGA1 4,220 mg/L
E. coli HGB6 4,940 mg/L
E. coli HGC4 6,170 mg/L
E. coli HGD3 6,500 mg/L
E. coli HGC3 6,590 mg/L
E. coli HGD 7,110 mg/L

Titer in Fed-Batch for L-tyrosine

Modifications
Strain Maximum production tyrREc aroGEc tyrAEc ppsAEc tktAEc tyrPEc aroLEc tyrCZm aroPEc aroHEc tnaAEc tnaBEc pheAEc pheLEc trpEEc dadXEc cvrAEc yddGEc ykgHEc betAEc sthAEc yeeJEc yeeLEc pntAEc pntBEc xfpkBa ptaEc poxBEc
E. coli T1 3,800 mg/L
E. coli T2 9,700 mg/L
E. coli BTY0.13 11,550 mg/L
E. coli BTY7.13 28,730 mg/L
E. coli BTY5.13 29,320 mg/L
E. coli BTY2.13 43,140 mg/L
E. coli HGC4 50,200 mg/L
E. coli DPD4193 55,000 mg/L
E. coli HGD 92,800 mg/L

Substrate yield in Fed-Batch for L-tyrosine

Modifications
Strain Maximum production tyrREc aroGEc tyrAEc ppsAEc tktAEc pheAEc trpEEc aroPEc tyrPEc dadXEc cvrAEc yddGEc ykgHEc betAEc sthAEc yeeJEc yeeLEc pntAEc pntBEc poxBEc xfpkBa ptaEc
E. coli T1 0.037 g/g of sustrate
E. coli T2 0.102 g/g of sustrate
E. coli HGD 0.278 g/g of sustrate

Substrate yield in Flask for L-tyrosine

Modifications
Strain Maximum production aroHEc tyrREc tnaAEc tnaBEc aroGEc tyrAEc pheAEc pheLEc trpEEc
E. coli DPD4193 0.09 g/g of sustrate

Biomass-specific yield in Flask for L-tyrosine

Modifications
Strain Maximum production ydiBEc aroDEc aroBEc aroGEc ppsAEc tktAEc tyrBEc tyrAEc aroCEc aroAEc aroKEc aroEEc aroLEc
E. coli A (pS0, pY0) 921 mg/g DCW
E. coli B (pS0, pY1) 944 mg/g DCW
E. coli E (pS4, pY2) 987 mg/g DCW
E. coli C (pS4, pY1) 1,307 mg/g DCW
E. coli D (pS5, pY1) 1,451 mg/g DCW
E. coli G (pS5, pY3) 1,617 mg/g DCW
E. coli F (pS4, pY3) 2,645 mg/g DCW