Titer in Flask for L-threonine

		Modifications
Strain	Maximum production	thrA	thrB	thrC	lacI	tdh	tdcC	rhtA	sstT	lysC	lysA	metA	ilvA
E. coli MG1655	16.55 mg/L
E. coli MDS42	19.61 mg/L
E. coli MG-102	34.06 mg/L
E. coli MDS-202	36.61 mg/L
E. coli MG-103	42.25 mg/L
E. coli MDS-203	79.94 mg/L
E. coli MG-104	138.01 mg/L
E. coli MG-105	165.54 mg/L
E. coli MDS-204	228.43 mg/L
E. coli MDS-205	303.23 mg/L
E. coli TH07(pBRThrABC)	10,100 mg/L

Titer in Batch for L-threonine

		Modifications
Strain	Maximum production	thrA	thrB	thrC	lacI	tdh	tdcC	rhtA	sstT	lysC	lysA	metA	ilvA	ppc	iclR	aceA	aceB	rhtC	rhtB
E. coli TH11C (pBRThrABC, pACYC177)	190 mg/L
E. coli TH07 (pBRThrABC, pACYCppc)	1,210 mg/L
E. coli TH07 (pBRThrABC, pACYC177)	1,460 mg/L
E. coli TH07(pBRThrABC)	1,480 mg/L
E. coli TH09C (pBRThrABC, pACYCaceBA)	1,740 mg/L
E. coli TH19C (pBRThrABC)	1,890 mg/L
E. coli TH09C (pBRThrABC, pACYC177)	1,920 mg/L
E. coli TH09C (pBRThrABC)	1,930 mg/L
E. coli TH20C (pBRThrABC)	6,390 mg/L
E. coli TH27C (pBRThrABC)	7,390 mg/L
E. coli TH27C (pBRThrABCR)	11,100 mg/L
E. coli TH27C (pBRThrABCR2)	11,590 mg/L
E. coli TH27C (pBRThrABCR3)	11,810 mg/L
E. coli MDS-205	40,100 mg/L

Titer in Fed-Batch for L-threonine

		Modifications
Strain	Maximum production	lacI	thrA	lysC	thrB	thrC	lysA	metA	ilvA	tdh	iclR	ppc	tdcC	rhtC	rhtA	rhtB	acs
E. coli TH27C (pBRThrABCR3)	77,100 mg/L
E. coli TH28C (pBRThrABCR3)	82,400 mg/L

Substrate yield in Batch for L-threonine

		Modifications
Strain	Maximum production	thrA	thrB	thrC	lacI	tdh	tdcC	rhtA	sstT	lysC	lysA	metA	ilvA	ppc	iclR	aceA	aceB	rhtC	rhtB
E. coli TH11C (pBRThrABC, pACYC177)	0.028 g/g of sustrate
E. coli TH07 (pBRThrABC, pACYCppc)	0.118 g/g of sustrate
E. coli TH07(pBRThrABC)	0.137 g/g of sustrate
E. coli TH07 (pBRThrABC, pACYC177)	0.14 g/g of sustrate
E. coli TH09C (pBRThrABC, pACYCaceBA)	0.169 g/g of sustrate
E. coli TH19C (pBRThrABC)	0.183 g/g of sustrate
E. coli TH09C (pBRThrABC, pACYC177)	0.186 g/g of sustrate
E. coli TH20C (pBRThrABC)	0.213 g/g of sustrate
E. coli TH27C (pBRThrABC)	0.246 g/g of sustrate
E. coli TH27C (pBRThrABCR)	0.37 g/g of sustrate
E. coli TH27C (pBRThrABCR2)	0.386 g/g of sustrate
E. coli TH27C (pBRThrABCR3)	0.393 g/g of sustrate
E. coli MDS-205	0.401 g/g of sustrate
E. coli TH09C (pBRThrABC)	0.86 g/g of sustrate

Substrate yield in Flask for L-threonine

		Modifications
Strain	Maximum production	lacI	thrA	lysC	thrB	thrC	lysA	metA	ilvA	tdh
E. coli TH07(pBRThrABC)	0.202 g/g of sustrate

Substrate yield in Fed-Batch for L-threonine

		Modifications
Strain	Maximum production	lacI	thrA	lysC	thrB	thrC	lysA	metA	ilvA	tdh	iclR	ppc	tdcC	rhtC	rhtA	rhtB	acs
E. coli TH27C (pBRThrABCR3)	0.275 g/g of sustrate
E. coli TH28C (pBRThrABCR3)	0.294 g/g of sustrate