Titer in Flask for L-methionine

		Modifications
Strain	Maximum production	metJ	metA	yjeH	metI	lysA
E. coli W3110	0 mg/L
E. coli W3110 ΔmetJ/pTrcA*	202.64 mg/L
E. coli W3110 ΔmetJ (pTrcA*H)	413.16 mg/L
E. coli W3110 ΔmetJΔmetI (pTrcA*H)	593.3 mg/L
E. coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H	4,080 mg/L

Titer in Fed-Batch for L-methionine

		Modifications
Strain	Maximum production	metJ	metI	lysA	metA	yjeH	thrB
E. coli W3110 ΔmetJΔmetIΔlysAΔthrB/pTrcA*H	4,090 mg/L
E. coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H	9,750 mg/L

Substrate yield in Batch for L-methionine

		Modifications
Strain	Maximum production	metJ	metI	metA	yjeH	lysA	thrB
E. coli W3110 ΔmetJΔmetIΔthrB/pTrcA*H	0.02 g/g of sustrate
E. coli W3110 ΔmetJΔmetI (pTrcA*H)	0.03 g/g of sustrate
E. coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H	0.19 g/g of sustrate
E. coli W3110 ΔmetJΔmetIΔlysAΔthrB/pTrcA*H	0.2 g/g of sustrate

Substrate yield in Fed-Batch for L-methionine

		Modifications
Strain	Maximum production	metJ	metI	lysA	metA	yjeH
E. coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H	0.14 g/g of sustrate

Volumetric productivity in Fed-Batch for L-methionine

		Modifications
Strain	Maximum production	metJ	metI	lysA	metA	yjeH
E. coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H	200 mg/L/h