Titer in Flask for L-isoleucine

		Modifications
Strain	Maximum production	lacI	thrA	lysC	thrB	thrC	lysA	metA	ilvA	tdh	iclR	ppc	ilvI	ilvH	ygaZ	ygaH	ilvE	ilvD	ilvC	lrp
E. coli ILE01 (pTacilvAIH, pBRthrABC)	322 mg/L
E. coli ILE01 (pTacilvAIH, pBRthrABCygaZH)	1,110 mg/L
E. coli ILE02 (pTacilvAIH, pBRthrABCygaZH)	2,110 mg/L
E. coli ILE03 (pTacilvAIH, pBRthrABCygaZH)	2,830 mg/L

Titer in Fed-Batch for L-isoleucine

		Modifications
Strain	Maximum production	lacI	thrA	lysC	thrB	thrC	lysA	metA	ilvA	tdh	iclR	ppc	ilvE	ilvD	ilvC	lrp	ilvI	ilvH	ygaZ	ygaH
E. coli ILE03 (pTacilvAIH, pBRthrABCygaZH)	9,460 mg/L

Volumetric productivity in Fed-Batch for L-isoleucine

		Modifications
Strain	Maximum production	lacI	thrA	lysC	thrB	thrC	lysA	metA	ilvA	tdh	iclR	ppc	ilvE	ilvD	ilvC	lrp	ilvI	ilvH	ygaZ	ygaH
E. coli ILE03 (pTacilvAIH, pBRthrABCygaZH)	158 mg/L/h

Substrate yield in Fed-Batch for L-isoleucine

		Modifications
Strain	Maximum production	lacI	thrA	lysC	thrB	thrC	lysA	metA	ilvA	tdh	iclR	ppc	ilvE	ilvD	ilvC	lrp	ilvI	ilvH	ygaZ	ygaH
E. coli ILE03 (pTacilvAIH, pBRthrABCygaZH)	0.14 g/g of sustrate