Titer in Flask for L-cysteine

Modifications
Strain Maximum production cysE eamA tnaA sdaA serA serB serC cyuA yhaO decR cysM nrdH cysK glpE cysB
E. coli MG1655 (pMIV-cysE5, pACYC-yhaM1) 14.9 mg/L
E. coli MG1655 (pMIV-cysE5) 64.6 mg/L
E. coli MG1655 (pMIV-cysE5, pACYC177) 74.6 mg/L
E. coli LH09 85.21 mg/L
E. coli LH15 155.26 mg/L
E. coli LH03 190.3 mg/L
E. coli MG1655 ΔyhaM (pMIV-cysE5) 316.2 mg/L
E. coli LH04 492 mg/L
E. coli LH05 547.3 mg/L
E. coli LH13 550.35 mg/L
E. coli LH07 560.21 mg/L
E. coli LH06 620.92 mg/L
E. coli LH08 679.41 mg/L
E. coli LH14 742.92 mg/L
E. coli LH10 816.36 mg/L
E. coli LH11 968.22 mg/L
E. coli LH12 1,035.63 mg/L
E. coli LH16 1,359.12 mg/L

Titer in Fed-Batch for L-cysteine

Modifications
Strain Maximum production tnaA sdaA serA serB serC cyuA cysM nrdH cysE eamA cysB
E. coli LH06 5,100 mg/L
E. coli LH16 7,500 mg/L

Substrate yield in Flask for L-cysteine

Modifications
Strain Maximum production tnaA sdaA serA serB serC cysE eamA cyuA
E. coli MG1655 (pMIV-cysE5, pACYC-yhaM1) 0.00037 g/g of sustrate
E. coli MG1655 (pMIV-cysE5) 0.00164 g/g of sustrate
E. coli MG1655 (pMIV-cysE5, pACYC177) 0.00187 g/g of sustrate
E. coli MG1655 ΔyhaM (pMIV-cysE5) 0.0081 g/g of sustrate
E. coli LH03 0.018 g/g of sustrate
E. coli LH04 0.047 g/g of sustrate
E. coli LH05 0.053 g/g of sustrate
E. coli LH06 0.06 g/g of sustrate