Titer in Flask for fumarate

		Modifications
Strain	Maximum production	iclR	fumC	fumA	fumB	ppc	arcA	ptsG	aspA	lacI
E. coli CWF4	1,450 mg/L
E. coli CWF41	4,090 mg/L
E. coli CWF51	4,900 mg/L
E. coli CWF611	5,060 mg/L
E. coli CWF61	5,340 mg/L
E. coli CWF81	5,850 mg/L
E. coli CWF811	6,250 mg/L

Titer in Batch for fumarate

		Modifications
Strain	Maximum production	iclR	fumC	fumA	fumB	arcA	ptsG	aspA	lacI	ppc	galP
E. coli CWF812	7,850 mg/L
E. coli CWF811	8,230 mg/L

Titer in Fed-Batch for fumarate

		Modifications
Strain	Maximum production	iclR	fumC	fumA	fumB	arcA	ptsG	aspA	lacI	ppc	galP
E. coli CWF811	26,200 mg/L
E. coli CWF812	28,200 mg/L

Substrate yield in Flask for fumarate

		Modifications
Strain	Maximum production	iclR	fumC	fumA	fumB	ppc	arcA	ptsG	aspA	lacI
E. coli CWF4	0.097 g/g of sustrate
E. coli CWF41	0.273 g/g of sustrate
E. coli CWF51	0.327 g/g of sustrate
E. coli CWF611	0.343 g/g of sustrate
E. coli CWF61	0.35 g/g of sustrate
E. coli CWF81	0.41 g/g of sustrate
E. coli CWF811	0.417 g/g of sustrate

Substrate yield in Batch for fumarate

		Modifications
Strain	Maximum production	iclR	fumC	fumA	fumB	arcA	ptsG	aspA	lacI	ppc	galP
E. coli CWF812	0.394 g/g of sustrate
E. coli CWF811	0.415 g/g of sustrate

Substrate yield in Fed-Batch for fumarate

		Modifications
Strain	Maximum production	iclR	fumC	fumA	fumB	arcA	ptsG	aspA	lacI	ppc	galP
E. coli CWF811	0.361 g/g of sustrate
E. coli CWF812	0.389 g/g of sustrate

Volumetric productivity in Batch for fumarate

		Modifications
Strain	Maximum production	iclR	fumC	fumA	fumB	arcA	ptsG	aspA	lacI	ppc	galP
E. coli CWF811	305 mg/L/h
E. coli CWF812	393 mg/L/h

Volumetric productivity in Fed-Batch for fumarate

		Modifications
Strain	Maximum production	iclR	fumC	fumA	fumB	arcA	ptsG	aspA	lacI	ppc	galP
E. coli CWF811	252 mg/L/h
E. coli CWF812	448 mg/L/h

Specific production rate in Batch for fumarate

		Modifications
Strain	Maximum production	iclR	fumC	fumA	fumB	arcA	ptsG	aspA	lacI	galP	ppc
E. coli CWF811	201 mg/g DCW/h
E. coli CWF812	238 mg/g DCW/h