Titer in Fed-Batch for (R)-citramalate

		Modifications
Strain	Maximum production	leuC	ackA	pta	poxB	gltA	cimA
E. coli MEC626/pZE12-cimA	60,000 mg/L

Titer in Flask for (R)-citramalate

		Modifications
Strain	Maximum production	leuC	ackA	pta	poxB	gltA	cimA
E. coli MEC624/pZE12-cimA	460 mg/L
E. coli MEC649/pZE12-cimA	840 mg/L
E. coli MEC648/pZE12-cimA	980 mg/L
E. coli MEC654/pZE12-cimA	1,230 mg/L
E. coli MEC613/pZE12-cimA	1,720 mg/L
E. coli MEC626/pZE12-cimA	1,810 mg/L

Titer in Batch for (R)-citramalate

		Modifications
Strain	Maximum production	leuC	ackA	pta	poxB	gltA	cimA
E. coli MEC613/pZE12-cimA	4,700 mg/L
E. coli MEC626/pZE12-cimA	11,000 mg/L

Substrate yield in Fed-Batch for (R)-citramalate

		Modifications
Strain	Maximum production	leuC	ackA	pta	poxB	gltA	cimA
E. coli MEC626/pZE12-cimA	0.53 g/g of sustrate

Substrate yield in Flask for (R)-citramalate

		Modifications
Strain	Maximum production	leuC	ackA	pta	poxB	gltA	cimA
E. coli MEC649/pZE12-cimA	0.4 g/g of sustrate
E. coli MEC624/pZE12-cimA	0.432 g/g of sustrate
E. coli MEC613/pZE12-cimA	0.47 g/g of sustrate
E. coli MEC648/pZE12-cimA	0.49 g/g of sustrate
E. coli MEC654/pZE12-cimA	0.5 g/g of sustrate
E. coli MEC626/pZE12-cimA	0.57 g/g of sustrate

Substrate yield in Batch for (R)-citramalate

		Modifications
Strain	Maximum production	leuC	ackA	pta	poxB	gltA	cimA
E. coli MEC626/pZE12-cimA	0.37 g/g of sustrate