Lihong Du, Zhen Zhang, Qingyang Xu & Ning Chen (2019). Central metabolic pathway modification to improve L-tryptophan production in Escherichia coli. Bioengineered.
Strains
- Escherichia coli TRP07
- Escherichia coli TRP06
- Escherichia coli TRP05
- Escherichia coli TRP04
- Escherichia coli TRP03
- Escherichia coli TRP02
- Escherichia coli TRP01
- Escherichia coli TRP1
- Escherichia coli W3110
Metabolite production
-
acetate
-
Titer
- 2.2 g/L (Escherichia coli TRP07 in Fed-Batch)
-
L-tryptophan
-
Titer
- 35.0 g/L (Escherichia coli TRP03 in Fed-Batch)
- 4.0 g/L (Escherichia coli TRP04 in Flask)
- 4.0 g/L (Escherichia coli TRP05 in Flask)
- 20.0 g/L (Escherichia coli TRP05 in Fed-Batch)
- 7.0 g/L (Escherichia coli TRP06 in Flask)
- 49.0 g/L (Escherichia coli TRP07 in Fed-Batch)
- 12.0 g/L (Escherichia coli TRP07 in Flask)
- 3.0 g/L (Escherichia coli TRP02 in Flask)
- 8.1 g/L (Escherichia coli TRP03 in Flask)
- 0.24 g/L (Escherichia coli TRP01 in Flask)
- 0.13 g/L (Escherichia coli TRP1 in Flask) Volumetric productivity
- 2.8 g/L/h (Escherichia coli TRP07 in Fed-Batch) Substrate yield
- 0.186 g/g of sustrate (Escherichia coli TRP07 in Fed-Batch)