Yu Deng, Yin Mao & Xiaojuan Zhang (2015). Metabolic engineering of E. coli for efficient production of glycolic acid from glucose. Biochemical Engineering Journal.

Strains

• Escherichia coli BW25113-pGLY-1
• Escherichia coli EXY-1
• Escherichia coli EXY-1-pGLY-1
• Escherichia coli EYX-1-glcB-
• Escherichia coli EYX-1-glcb-1-pGLY-1
• Escherichia coli EYX-1WAK
• Escherichia coli EYX-2
• Escherichia coli BW25113

Plasmids

• pGLY-1
• pCOLADuet-1

Metabolite production

• glycolate
  Substrate yield
  • 0.33 g/g of sustrate (Escherichia coli EYX-2 in Flask)
  • 0.0813 g/g of sustrate (Escherichia coli BW25113-pGLY-1 in Flask)
  • 0.11 g/g of sustrate (Escherichia coli EXY-1-pGLY-1 in Flask)
  • 0.48 g/g of sustrate (Escherichia coli EYX-2 in Batch)
  • 0.52 g/g of sustrate (Escherichia coli EYX-2 in Fed-Batch)
  Titer
  • 0.65 g/L (Escherichia coli BW25113-pGLY-1 in Flask)
  • 0.88 g/L (Escherichia coli EXY-1-pGLY-1 in Flask)
  • 2.44 g/L (Escherichia coli EYX-1-glcb-1-pGLY-1 in Flask)
  • 2.66 g/L (Escherichia coli EYX-2 in Flask)
  • 8.96 g/L (Escherichia coli EYX-2 in Batch)
  • 56.44 g/L (Escherichia coli EYX-2 in Fed-Batch)