Li, Qingchen; Li, Chenxi; Zhong, Jie; Wang, Yukun; Yang, Qinghua; Wang, Bingmei; He, Wenjin; Huang, Jianzhong; Lin, Shengyuan & Qi, Feng. Metabolic engineering of Escherichia coli for N-methylserotonin biosynthesis. Metabolic Engineering. 2025, 87, 49-59.
Strains
- Escherichia coli DH5α
- Escherichia coli MGΔtnaA
- Escherichia coli MGΔtnaAΔnudB
- Escherichia coli MGΔtnaAΔqueD
- Escherichia coli MGΔtnaAΔqueDΔnudB
- Escherichia coli MGΔtnaAΔmetJ
- Escherichia coli MGΔtnaAΔqueDΔmetJ
- Escherichia coli HTP-1
- Escherichia coli HTP-2
- Escherichia coli HTP-3
- Escherichia coli HTP-4
- Escherichia coli HTP-5
- Escherichia coli HTP-6
- Escherichia coli HTP-7
- Escherichia coli HTP-8
- Escherichia coli HTP-9
- Escherichia coli HTP-10
- Escherichia coli HTP-11
- Escherichia coli HTP-12
- Escherichia coli HTP-13
- Escherichia coli HTP-14
- Escherichia coli HTP-15
- Escherichia coli HTP-16
- Escherichia coli HTP-17
- Escherichia coli HT1
- Escherichia coli NMS-0
- Escherichia coli NMS-1
- Escherichia coli NMS-2
- Escherichia coli NMS-3
- Escherichia coli NMS-4
- Escherichia coli NMS-5
- Escherichia coli NMS-6
- Escherichia coli NMS-7
- Escherichia coli NMS-8
- Escherichia coli NMS-9
- Escherichia coli NMS-10
- Escherichia coli NMS-11
- Escherichia coli NMS-12
- Escherichia coli NMS-13
- Escherichia coli NMS-14
- Escherichia coli NMS-15
- Escherichia coli NMS-16
- Escherichia coli NMS-17
- Escherichia coli NMS-18
- Escherichia coli NMS-19
- Escherichia coli NMS-20
- Escherichia coli MG1655
Plasmids
- pTrc99A
- pCold-ProS2
- pTrc-TPH
- pTrc-TE
- pTrc-TX
- pTrc-TM
- pTrc-TB
- pTrc-TBE
- pTrc-TBM
- pTrc-TBEX
- pTrc-TBEM
- pTrc-TBEMX
- pTrc-TBZmEMX
- pTrc-TBSgEMX
- pTrc-TBBsEMX
- pTrc-BlINMT
- pTrc-HsINMT
- pTrc-NpINMT
- pTrc-TBX
- pTrc-EsPaNMT
- pTrc-HsPeNMT
- pTrc-AtPNMT
- pTrc-RgANMT
- pTrc-BlI*
- pTrc-HsI*
- pTrc-NpI*
- pTrc-EsPa*
- pTrc-HsPe*
- pTrc-AtP*
- pTrc-RgA*
- pTrc-DHsI*ml
- pTrc-DHsI*mlS
- pACYC184
- pAC-DHsI*mlS
- pTrc-DHsI*
- pAC-TBBsEMX
Metabolite production
-
5-hydroxy-L-tryptophan
-
Titer
- 51.7 mg/L (Escherichia coli HTP-14 in Flask)
- 56.7 mg/L (Escherichia coli HTP-12 in Flask)
- 72.6 mg/L (Escherichia coli HTP-13 in Flask)
- 122.3 mg/L (Escherichia coli HTP-17 in Flask)
- 31.4 mg/L (Escherichia coli HTP-15 in Flask)
- 78.83 mg/L (Escherichia coli HTP-16 in Flask)
- 379.62 mg/L (Escherichia coli NMS-15 in Flask)
- 12.53 mg/L (Escherichia coli NMS-20 in Flask)
- 252.0 mg/L (Escherichia coli NMS-19 in Fed-Batch)
- 371.5 mg/L (Escherichia coli NMS-19 in Fed-Batch)
- 23.53 mg/L (Escherichia coli NMS-19 in Flask)
- 18.7 mg/L (Escherichia coli HTP-1 in Flask)
- 27.5 mg/L (Escherichia coli HTP-2 in Flask)
- 24.0 mg/L (Escherichia coli HTP-3 in Flask)
- 23.7 mg/L (Escherichia coli HTP-4 in Flask)
- 23.1 mg/L (Escherichia coli HTP-5 in Flask)
- 29.9 mg/L (Escherichia coli HTP-6 in Flask)
- 22.5 mg/L (Escherichia coli HTP-7 in Flask)
- 22.3 mg/L (Escherichia coli HTP-8 in Flask)
- 30.03 mg/L (Escherichia coli HTP-9 in Flask)
- 41.35 mg/L (Escherichia coli HTP-10 in Flask)
- 57.2 mg/L (Escherichia coli HTP-11 in Flask)
-
N-Methylserotonin
-
Titer
- 8.7 mg/L (Escherichia coli NMS-15 in Flask)
- 10.08 mg/L (Escherichia coli NMS-16 in Flask)
- 4.21 mg/L (Escherichia coli NMS-20 in Flask)
- 128.6 mg/L (Escherichia coli NMS-19 in Fed-Batch)
- 5.18 mg/L (Escherichia coli NMS-19 in Flask)
- 19.36 mg/L (Escherichia coli NMS-17 in Flask)
- 25.4 mg/L (Escherichia coli NMS-18 in Flask)
- 6.5 mg/L (Escherichia coli NMS-14 in Flask)
- 8.45 mg/L (Escherichia coli NMS-13 in Flask)
- 7.26 mg/L (Escherichia coli NMS-12 in Flask)
- 7.95 mg/L (Escherichia coli NMS-11 in Flask)
- 9.06 mg/L (Escherichia coli NMS-10 in Flask)
- 12.1 mg/L (Escherichia coli NMS-9 in Flask)
- 7.84 mg/L (Escherichia coli NMS-8 in Flask)
- 1.13 mg/L (Escherichia coli NMS-7 in Flask)
- 1.02 mg/L (Escherichia coli NMS-6 in Flask)
- 0.85 mg/L (Escherichia coli NMS-5 in Flask)
- 1.36 mg/L (Escherichia coli NMS-4 in Flask)
- 1.36 mg/L (Escherichia coli NMS-3 in Flask)
- 1.6 mg/L (Escherichia coli NMS-2 in Flask)
- 1.36 mg/L (Escherichia coli NMS-1 in Flask)
-
serotonin
-
Titer
- 17.73 mg/L (Escherichia coli NMS-15 in Flask)
- 9.9 mg/L (Escherichia coli NMS-20 in Flask)
- 20.41 mg/L (Escherichia coli NMS-19 in Flask)
- 16.23 mg/L (Escherichia coli NMS-16 in Flask)
- 14.09 mg/L (Escherichia coli NMS-17 in Flask)
- 13.97 mg/L (Escherichia coli NMS-18 in Flask)