Sangkyu Park, Kiyoon Kang, Shin Woo Lee, Mi-Jeong Ahn, Jung-Myung Bae & Kyoungwhan Back (2010). Production of serotonin by dual expression of tryptophan decarboxylase and tryptamine 5-hydroxylase in Escherichia coli. Applied Microbiology and Biotechnology.

Strains

• Escherichia coli BL21(DE3)
• Escherichia coli BL21(DE3)/pET300-T5H
• Escherichia coli BL21(DE3)/pCOLADuet-GST∆37T5H+TDC
• Escherichia coli BL21(DE3)/pET300-∆37T5H-RR

Plasmids

• pCOLADuet-1
• pET300/NT-DEST
• pET300-T5H
• pCOLADuet-GST∆37T5H+TDC
• pET300-∆37T5H-RR

Metabolite production

• serotonin
  Titer
  • 0.0 g/L (Escherichia coli BL21(DE3)/pET300-T5H in Flask)
  • 0.0 g/L (Escherichia coli BL21(DE3)/pET300-∆37T5H-RR in Flask)
  • 24.0 mg/L (Escherichia coli BL21(DE3)/pCOLADuet-GST∆37T5H+TDC in Flask)