Yanan Hao, Qian Ma, Xiaoqian Liu, Xiaoguang Fan, Jiaxuan Men, Heyun Wu, Shuai Jiang, Daoguang Tian, Bo Xiong & Xixian Xie (2020). High-yield production of L-valine in engineered Escherichia coli by a novel two-stage fermentation. Metabolic Engineering.

Strains

• Escherichia coli VHY01
• Escherichia coli VHY03
• Escherichia coli VHY04
• Escherichia coli VHY05
• Escherichia coli VHY06
• Escherichia coli VHY07
• Escherichia coli VHY08
• Escherichia coli VHY09
• Escherichia coli VHY10
• Escherichia coli VHY11
• Escherichia coli VHY12
• Escherichia coli VHY13
• Escherichia coli VHY14
• Escherichia coli VHY15
• Escherichia coli VHY16
• Escherichia coli VHY17
• Escherichia coli VHY18
• Escherichia coli VHY02
• Escherichia coli W3110

Metabolite production

• L-valine
  Titer
  • 86.0 g/L (Escherichia coli VHY18 in Fed-Batch)
  • 84.0 g/L (Escherichia coli VHY18 in Fed-Batch)
  • 0.0 g/L (Escherichia coli W3110 in Flask)
  • 0.3 g/L (Escherichia coli VHY01 in Flask)
  • 4.65 g/L (Escherichia coli VHY02 in Flask)
  • 10.5 g/L (Escherichia coli VHY03 in Flask)
  • 10.5 g/L (Escherichia coli VHY04 in Flask)
  • 16.53 g/L (Escherichia coli VHY05 in Flask)
  • 18.6 g/L (Escherichia coli VHY06 in Flask)
  • 19.125 g/L (Escherichia coli VHY07 in Flask)
  • 19.0 g/L (Escherichia coli VHY08 in Flask)
  • 18.875 g/L (Escherichia coli VHY09 in Flask)
  • 32.0 g/L (Escherichia coli VHY10 in Flask)
  • 31.875 g/L (Escherichia coli VHY11 in Flask)
  • 25.77 g/L (Escherichia coli VHY12 in Flask)
  • 39.1 g/L (Escherichia coli VHY13 in Flask)
  • 41.2 g/L (Escherichia coli VHY14 in Flask)
  • 41.3 g/L (Escherichia coli VHY15 in Flask)
  Substrate yield
  • 0.33 g/g of sustrate (Escherichia coli VHY18 in Fed-Batch)
  • 0.41 g/g of sustrate (Escherichia coli VHY18 in Fed-Batch)
  • 0.043 g/g of sustrate (Escherichia coli VHY02 in Flask)
  • 0.1 g/g of sustrate (Escherichia coli VHY03 in Flask)
  • 0.1 g/g of sustrate (Escherichia coli VHY04 in Flask)
  • 0.146 g/g of sustrate (Escherichia coli VHY05 in Flask)
  • 0.17 g/g of sustrate (Escherichia coli VHY06 in Flask)
  • 0.195 g/g of sustrate (Escherichia coli VHY07 in Flask)
  • 0.194 g/g of sustrate (Escherichia coli VHY08 in Flask)
  • 0.191 g/g of sustrate (Escherichia coli VHY09 in Flask)
  • 0.3 g/g of sustrate (Escherichia coli VHY10 in Flask)
  • 0.3 g/g of sustrate (Escherichia coli VHY11 in Flask)
  • 0.19 g/g of sustrate (Escherichia coli VHY12 in Flask)
  • 0.32 g/g of sustrate (Escherichia coli VHY13 in Flask)
  • 0.33 g/g of sustrate (Escherichia coli VHY14 in Flask)
  • 0.33 g/g of sustrate (Escherichia coli VHY15 in Flask)
  Volumetric productivity
  • 1.95 g/L/h (Escherichia coli VHY18 in Fed-Batch)
  • 2.33 g/L/h (Escherichia coli VHY18 in Fed-Batch)