Min Liu, Jiali Lou, Jiali Gu, Xiao-Mei Lyu, Feng-Qing Wang & Dong-Zhi Wei (2020). Increasing L-homoserine production in Escherichia coli by engineering the central metabolic pathways. Journal of Biotechnology.

Strains

• Escherichia coli LJL1
• Escherichia coli LJL2
• Escherichia coli LJL3
• Escherichia coli LJL4
• Escherichia coli LJL5
• Escherichia coli LJL6
• Escherichia coli LJL7
• Escherichia coli LJL8
• Escherichia coli LJL9
• Escherichia coli LJL10
• Escherichia coli LJL11
• Escherichia coli LJL12
• Escherichia coli W3110

Plasmids

• pKK-miniPtac
• pKK-miniPtac-thrA

Metabolite production

• L-homoserine
  Titer
  • 0.2 g/L (Escherichia coli LJL3 in Flask)
  • 0.5 g/L (Escherichia coli LJL8 in Flask)
  • 0.8 g/L (Escherichia coli LJL9 in Flask)
  • 1.7 g/L (Escherichia coli LJL10 in Flask)
  • 2.1 g/L (Escherichia coli LJL11 in Flask)
  • 0.0 g/L (Escherichia coli W3110 in Flask)
  • 3.2 g/L (Escherichia coli LJL12 in Flask)
  • 35.8 g/L (Escherichia coli LJL12 in Fed-Batch)
  Volumetric productivity
  • 0.82 g/L/h (Escherichia coli LJL12 in Fed-Batch)
  Substrate yield
  • 0.35 g/g of sustrate (Escherichia coli LJL12 in Flask)
• acetate
  Titer
  • 6.9 g/L (Escherichia coli LJL12 in Fed-Batch)
  Substrate yield
  • 0.069 g/g of sustrate (Escherichia coli LJL12 in Fed-Batch)